Andrei Chistoserdov

1980 B.S. (Biochemistry) Moscow State University, Moscow, Russia

1982 M.S. (Biochemistry) Moscow State University, Moscow, Russia

1985 Ph.D. (Microbial Genetics) Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia

My primary scientific interests include different aspects of microbial physiology and applied microbiology. I am particularly interested in the physiology and genetics of lipid metabolism in oleaginous bacteria such as Acinetobacter baylyi. A. baylyi possesses multiple pathways for lipid utilization, functions of which are still poorly understood. Recently, my laboratory began to work with several genera of clostridia-like microorganisms with the goal of understanding the regulation mechanisms and optimization of hydrogen production in these bacteria. The primary focus of my applied microbiology research is on the development of biofuels (biodiesel, methane and hydrogen gas) producers. To achieve this goal, we work with individual microorganisms and microbial communities, with a particular emphasis on fermentative anaerobes. In order to understand the dynamics of these communities, we use an array of microbial ecology methods such as 16S rRNA gene library analysis, real-time PCR, metagenomcs and metatranscriptomics. Several other microbial ecology projects in my laboratory were focused on the understanding of the role of microorganisms in the biogeochemistry of aquatic sediments and diseases of aquatic animals. To address these scientific probelms, my students and I use both cultivation-dependent (enrichment and pure cultures) and cultivation-independent (16S rDNA libraries/metagenomic/metatransciptomis and metabolomics) techniques.

Biodiesel production using cheap feeding stocks such as plant biomass hydrolysates is the primary project in my laboratory, which is tightly linked with understanding of lipid metabolism. We are developing a community of microorganisms capable of utilizing all hydrolysate components – glucose, xylose and even lignin. A recent project, funded by the NASA, was focused on molecular hydrogen and methane production on Mars through utilization of human wastes. Both projects have been conducted in a close collaboration with the Chemical Engineering Department of our University, which substantially increases the chances for the development of commercially viable technologies. We continue this project with pure cultures of both oleaginous and hydrogen-producing microorganisms such as bacteria and yeasts. Another project in my laboratory related to planet exploration is dealing with the biology of cyanobacteria, which can be potentially be used for oxygen production on the Moon and Mars. Specific questions include cyanobacterial adaptations to iron toxicity, exopolysaccharide biosynthesis and photosynthesis mechanisms including those for far-red photosynthesis. My earlier microbial ecology projects include elucidating the role of microorganisms in the coupling of the carbon and sulfur cycles at oxic-anoxic interfaces is the major microbial ecology project in my laboratory. Originally, my research was focused on two distinctive marine environments: the Cariaco Basin, the world's second largest permanently anoxic basin and mobile tropical deltaic muds characterized by physically reworked layers dominated by suboxic processes. Environmental metagenomics is a new direction for microbial ecology, bacterial genomics and me. Its goal is to characterize genomes of environmentally important yet non-cultivable bacteria, elucidate what genes are expressed in any given environmental condition and investigate how the flow of nutrients between the members of the community takes place. Metagenomics data will demonstrate a full metabolic potential of microorganisms in question and indicate their possible involvement in biogeochemical processes in their environment.

Graduate student projects are expected to be framed in agreement with the general directions of the research in my laboratory. Although the work of a microbiologist is generally in the laboratory, those students who are interested in microbial ecology will be going to the field for collection of samples. Students interested in applied microbiology will work in close collaboration with students and faculty of the Chemical Engineering Department. Graduate students are expected to publish project results in peer-reviewed journals and present data at professional conferences.

Selected publications:

· Deb Adhikary, N.R., P.L. Klerks, and A.Y. Chistoserdov. 2024. Bacterial community composition in the northern Gulf of Mexico intertidal sediment bioturbated by the ghost shrimp Lepidophthalmus louisianensis. Antonie van Leeuwenhoek. 117: 21. doi 10.1007/s10482-023-01897-y

· Revellame, E.D., R. Aguda, K.M. Gatdula, W. Holmes, D.L. Fortela, W. Sharp, D. Gang, A. Chistoserdov, R. Hernandez and M.E. Zappi. 2024. Microalgae in bioregenerative life support systems for space applications. Algal Research 77(1): 103332.

· Luo, L., A.P. Martin, E.K. Tandoh, A. Chistoserdov, L.V. Slipchenko, S. Savikhin and W. Xu. 2024. Impact of Peripheral Hydrogen Bond on Electronic Properties of the Primary Acceptor Chlorophyll in the Reaction Center of Photosystem I. Int. J. Molec. Sci. 25(9): 4815. https://doi.org/10.3390/ijms25094815

· Quinn, R.A., S. Hazra, R. Smolowitz and A.Y. Chistoserdov. 2017. Real-time PCR assay for Aquimarina macrocephali subsp. homaria and its distribution in shell disease lesions of Homarus americanus, Milne-Edwards, 1837, and environmental samples. J. Microbiol. Meth. 139:61-67.

· Ukey, R., W. Holmes, R. Bajpai, and A.Y. Chistoserdov. 2017. Evaluation of thioesterases from Acinetobacter baylyi for production of free fatty acids. Can. J. Microbiol. 63(4):321-329. doi: 10.1139/cjm-2016-0458.

· Rodriguez-Mora M.J., V.P. Edgcomb, C. Taylor, M.I. Scranton, G.T. Taylor and A.Y. Chistoserdov. 2016. The diversity of sulfide oxidation and sulfate reduction genes expressed by the bacterial communities of the Cariaco Basin, Venezuela. Open Microbiol. J. 10: BSP-TOMICROJ-2016-28. doi: 10.2174/1874285801610010140.

· Fortela, D.L., R. Hernandez, A. Chistoserdov, M. Zappi, R. Bajpai, D. Gang, E. Revellameand and W. Holmes. 2016. Biodiesel Profile Stabilization and Microbial Community Selection of Activated Sludge Feeding on Acetic Acid as a Carbon Source. ACS Sustainable Chem. Eng., 4(12): 6427–6434.

· Fortela, D.L., R. Hernandezi, M. Zappi, T.W. French, R. Bajpai, A. Chistoserdov, E. Revellame and W. Holmes. 2016. Microbial lipid accumulation capability of activated sludge feeding on short chain fatty acids as carbon sources through fed-batch cultivation. J. Bioprocess Biotech 6: 275. doi:10.4172/2155-9821.1000275.

· Liu, J.-Z., W. Xu, A. Chistoserdov, R.K. Bajpai. 2016. Glycerol Dehydratases: Biochemical Structures, Catalytic Mechanisms, and Industrial Applications in 1,3-Propanediol Production by Naturally-Occurring and Genetically-Engineered Bacterial Strains. Appl. Biochem. Biotechnol. 179(6):1073-1100. DOI: 10.1007/s12010-016-2051-6.

· Luzan, T and A.Y. Chistoserdov. 2016. Characterization of Enrichment Cultures Involved in the Carbon, Sulfur and Iron Biogeochemical Cycles in French Guiana Mobile Muds. Geomicrobiol. J. doi: 10.1080/01490451.2015.1137656.

· Rodriguez-Mora M.J., M.I. Scranton, G.T. Taylor, and A.Y. Chistoserdov. 2015. Bacterial Diversity in the Redox Transition and Anoxic Zones of the Cariaco Basin Assessed by Massively Parallel Tag Sequencing. FEMS Microbiol. Ecol. 91(9): fiv088.

Feel free to contact me at this address:

Andrei Chistoserdov, Department of Biology, PO Box 42451, Lafayette, LA 70504 Telephone: (337) 482- 1330